The DNA methylome is an incredibly rich signature of cell identity and gene regulation. But what if we're missing half the story? Our new preprint shows that, for neurons, 5-methyl-cytosine (5mC) is only the tip of the methylberg... 🧵

Exciting to see a package of brain development studies (mainly from mouse) from the NIH (not on bsky!?) #BRAINInitiative . Congrats to the authors, and I'm looking forward to digging into the findings/data. www.nature.com/collections/...

What do you get when you cross a joke with a rhetorical question?

I have been trying to get this published as an op-ed, but I am going to post it here since I think it is timely in light of the "consent" extortion events. Deafening Quiet from the Scientific Establishment jeremymberg.github.io/jeremyberg.g... 1/14

🧪 And House appropriators say: no cuts to NIH budget! House subcommittee version just came out (link below). Holds NIH flat at $48 billion. To be clear: this is far from over. But a good sign that science still matters across both sides of aisle. YOUR VOICE MATTERS!!

I always enjoyed visiting DC for neuroscience conferences, but now that they've sent in the storm troopers I'm sure the criminal violations of statistical best practices will finally end

Can universities go on strike by withholding licensing of all their patents by any US govt agency (incl military)? Seems like the only leverage they have and better than just giving their lunch money to the bullies

Interesting to see Nature retracting a study based on apparent over-clustering of single cell sequencing data www.thetransmitter.org/cell-types/n...

A reanalysis of data published in a 2024 Nature paper highlights a “thorny problem” in genetics research: how to balance curiosity and caution when searching for gene candidates. By Katie Moisse #neuroskyence www.thetransmitter.org/statistics/m...

Cool work, Marcelo et al!

I wrote a review of a recent paper on false discovery and multiple testing correction. liorpachter.wordpress.com/2025/06/16/r...

Great to see attention being paid to rigor in neuroscience methods.

My statement on what's unfolding in Los Angeles.

Always pseudobulk, always correct for multiple comparisons, and always consult with a biostatistian. www.nature.com/articles/s41...

www.nature.com/articles/s41... The engram is still out there, waiting to be rigorously discovered!

Very happy to see our comment about scRNAseq methods and rigor finally published. Now more than ever, science must be an example of integrity and self-examination. If we abandon rigor, we will be even more isolated and vulnerable.

Hard to process the news about Harvard and international students. Other universities should stand in solidarity with our colleagues who are being persecuted.

Francis Crick speculated that DNA modifications might be key to memory.... but synaptic molecules are specific to individual neural connections

Genuine question: what will all the talented US and international people who would have gone into science do instead? Real estate?

"A report by economists at American University in Washington DC estimates that a 50% reduction in federal science funding would reduce the US gross domestic product by approximately 7.6%." Science is an amazing engine of economic activity and progress for the US ... or at least it was.

First they came for the data, and I did not speak out... because I was not data Then they came for the models, and I did not speak... because I was not a model. Then they came for the scientists, and there was no one left to speak for me.

Science not Silence. (Kudos to the students on my hallway who put these up)

I signed and urge others to sign as well.

I'm ashamed of my country

So who is leading the resistance? We have no time to lose.

Spent today hearing inspiring and exciting science from fellow members of an NIH Consortium studying HIV and opioid effects on the 🧠. A reminder of what we have to lose if the US abandons our scientific enterprise.

So when we "buy" Greenland, will we call it Trumpistan? Donaldland? Maganesia? The Panama Canal will be called Trump's Trickle.

My awesome colleague @seantrott.bsky.social quoted on understanding the nature of our new robot overlords in @nytimes.com www.nytimes.com/2024/12/16/o...

Low tide in La Jolla today

Calcium imaging of Climbing Fiber complex spikes in mice performing skilled movements... Ahh this brings back fond memories for me :). Very cool.

What's the best response to bullshit Truth posts? Send a BS (bluesky) about it right here. (Skeet is not doing it for me)

I used to think the US was a country that valued and invested in science. Not sure how to keep up a brave face and try to inspire/encourage the next generation when we're nominating antiscience quacks to lead NIH.

Gene regulation involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. Our paper in Nature Genetics describes ChIP-DIP, a method for genome-wide mapping of hundreds of DNA-protein interactions in a single experiment. www.nature.com/articles/s41...

Today I learned that the Watson & Crick 1953 paper was not peer reviewed. Editor just published it. Good thing too, because Reviewer 2 would have objected to the small sample size (n=2 helices)