Diego Hernández-Saavedra (@dhdzsaavedra.bsky.social)

Finally here!! So excited to share my lab’s first paper. Clay did an amazing job trying to define how the muscle memory of exercise supports growth 💪🏼💪🏼💪🏼📈 #muscle #mitochondria @apspublications.bsky.social journals.physiology.org/doi/abs/10.1...

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Muscle Memory of Exercise Optimizes Mitochondrial Metabolism to Support Skeletal Muscle Growth | American Journal of Physiology-Cell Physiology | American Physiological Society Exercise protects against age-related declines in skeletal muscle mass and function while improving overall health. Exercise can also prime long-term muscle health to enhance adaptations upon exercise retraining, a phenomenon termed muscle memory that remains largely understudied. To assess how prior endurance training elicits a lasting metabolic memory in skeletal muscle, we utilized C57BL/6 mice fed either a control (CD) or obesogenic diet (HFD) that underwent 4-week training, detraining, and retraining periods. Our results show that exercise retraining attenuated weight gain and potentiated muscle growth, even with reduced voluntary running volumes. Training increased fiber size (fCSA), which disappeared with detraining and was recovered with retraining regardless of diet, pointing to a glycolytic-to-oxidative fiber shift. Transcriptomic analysis (bulk RNA-seq) of the retrained muscle revealed a robust enhancement of mitochondrial oxidative phosphorylation (OxPhos) and mitoribosomal genes, paralleled by increases in OxPhos protein complex IV levels, higher long-chain fatty acid oxidative capacity (ACADL), and sustained citrate synthase activity 1 week after retraining, reinforcing the optimization of mitochondrial metabolism. While transcriptomic evidence revealed a major overlap between HFD- and CD-fed mice, discrepancies in protein abundance emerged, which point to an intricate regulation of mitochondrial programming that supports the muscle memory of growth. Our study identifies common and selective mechanisms by which the muscle memory of exercise overrides dietary challenges and promotes fiber hypertrophy, offering insight into potential mechanisms to leverage to promote healthy aging. https://journals.physiology.org/doi/abs/10.1152/ajpcell.00451.2025