One of my favourite winter birds, a redpoll, checking things out below. #Alberta #birds #birding 🪶🇨🇦

Old school markers, still strong 🟣 Calbindin labels double cone photoreceptors, bipolar cells, and ganglion cells. 🟢 Calretinin highlights horizontal cells, bipolar cells, and IPL stratification. Avian retina circuitry, clearly written in layers. #FluorescenceFriday #Neuroscience #Retina #Imaging

Two years since I first arrived at the beautiful #UniversityofSussex campus Joined as a #Postdoctoral Research Fellow in 2024. Grateful for the experiences, challenges, growth, and the journey so far. Cheers to what’s ahead.

Attended and presented a poster at the wonderful 2 day #ASABWinter2025 meeting in the beautiful city of #Edinburgh. A coming together of researchers asking how sensory worlds shape #AnimalBehaviour. A genuine sense of community across generations of animal ethologists. @asab.org

My parents, Retd Prof R K Seth and Retd Prof Dr Ranjana Seth, published a new research paper today. Even at 69, they continue to push scientific boundaries. Watching them work with such dedication is one of my biggest inspirations. Link: www.tandfonline.com/eprint/HRTTZ...

Revisiting some older material and this paper came up again during a discussion about viral tools in avian retina. Our study was part of the Rising Stars in Avian Physiology collection and showed that AAV2 9 can reliably reach and transduce avian photoreceptors. More in thread below..

Ending my #FluorescenceFriday with this beautiful avian retina image depicting the beautiful stratification in the avian eyes. This is where complexity meets art. #retina #avian #bird #plexiformlayer

#FluorescenceFriday A sky of light within ✨ This is the ganglion cell layer of the avian retina. The nerve fiber layer lies beneath like a soft green current, carrying quiet signals forward. Sometimes to see the universe with its beautiful scattered stars, one only has to look inside.

Turns out zebrafish cones are frenemies. Red/UV see and Green/Blue suppress...

Layers of vision, glowing green #FluorescenceFriday Here, #photoreceptors expressing a #glutamate biosensor mark the first step in visual processing, with a single inner-retina #neuron (likely amacrine) revealing deeper circuit activity before signals ever reach the brain. #Neuroscience #Microscopy

#FluorescenceFriday 🌿🔬 post: Avian retina transduced with my AAVs carrying the #GluSNFR biosensor. Bright blobs at the top mark photoreceptor terminals; meanwhile below, an RGC connects beautifully with bipolar cells, giving a glimpse of retinal circuitry in action. #Retina #Neuroscience #AAV #2-P

Thrilled to share that my paper “AAV-mediated transduction of songbird retina” (Frontiers in Physiology, 19 Mar 2025) is featured in the official Rising Stars in Avian Physiology 2024 e-book! 🐦🔬 🔗 www.frontiersin.org/research-top... #AvianPhysiology #FrontiersInPhysiology

Day 2 #Visionarium2025 @teuler.bsky.social talks about What is a cell type?

Presented my poster “AAV-based Strategy for 2P Imaging in the Avian Retina” at #ERM2025 in Pécs 🇭🇺. I am grateful for inspiring discussions on retinal circuitry, photoreceptors, regeneration & artificial vision, and for connecting with colleagues in #RetinaResearch & #Neuroscience.

✨🔬 #FluorescenceFriday Confocal stack of chicken retina 🐥 — from inner nerve fibers up to photoreceptors. Layer upon layer of mosaics, each cell type in its place. Birds have remarkable visual systems, and under the microscope, they look absolutely stunning in detail.

How do birds see the world — and how can we study it at the cellular level? 🐦 Six months ago, our paper came out in Frontiers in Physiology with a new tool to answer exactly that. Here’s a little thread...🧵

Read up this brilliant paper by @gkafetzis.bsky.social on how retina evolution is so conserved.. or is it? 🤓

#FluorescenceFriday #Seahorse retina stained with #GNB3 (green) + #DAPI (cyan). GNB3 labels cone photoreceptors + ON #bipolarcells, tracing pathways from outer to inner retina. Amazing to see conserved visual #circuits in this non-traditional model

#FluorescenceFriday The #seahorse #retina shows off its beauty — stunning stratification in the inner nuclear and inner #plexiform layers, with rod #bipolar terminals forming a brilliant band. #Vertebrate retinas: the same basic plan, yet each species adds its own twist. Its so fascinating.

Seahorse retina in full color! This is a whole-mount of the seahorse #retina, labeled with Calretinin, Calbindin, and PKCα revealing its intricate architecture. Each marker highlights different neural circuits, showing how these remarkable creatures process the world around them. #FluorescenceFriday

#FluorescenceFriday Throwback to my #PhD: EGFP-labeled photoreceptors in a #Europeanrobin retina after AAV2/9 delivery Rods, cones, even a tiny horizontal cell. Lots of trial and error—but we got there. First of its kind in #songbirds. 📄 doi.org/10.3389/fphy... Still proud of it.

#MicroscopyMonday AAV injections in chick retina: #photoreceptors transduced as expected—plus widespread #Müllerglia labeling across layers! Shows AAV’s promise for multi-cell targeting in vision repair. 📄 doi.org/10.3389/fphy... #AAV #Retina #GeneTherapy

✨ #FluorescenceFriday update (coming early—conference-bound!)✨ Here’s a look at the stunning stratification of Photoreceptors in the chicken retina, captured using immunostaining and AAV-mediated gene delivery. 🟢 GFP, 🔴 PSD95, 🔵 DAPI It’s gratifying to see the biology light up like this—literally.

Orthogonal slice of a confocal Z-stack from a whole-mounted #avian #retina, highlighting the laminar relationship between the nerve fiber layer ( #NFL ) and underlying #RGCs. Imaged with Leica #SP8, processed in Fiji. #FluorescenceFriday #BirdRetina #Neuroscience #ConfocalImaging

Here is a cool post for #MicroscopyMonday. Here's a 3D visualization from my recent project using #SP8 confocal #microscopy. Red = DAPI nuclear staining Green = Photoreceptor transduction via AAV serotypes with reporter/ #biosensor genes This image shows successful targeting of photoreceptors.

#FluorescenceFriday 🐥:3D projection of #GluSnFR expression in #chick #retina following #AAV transduction. Expression is concentrated in the #photoreceptor layer. 🟢 GluSnFR (glutamate sensor) 🔴 DAPI-stained nuclei #Imaging #AAV #Retina #Biosensor #neuron #vision (doi.org/10.3389/fphys.2025.1549585)

The beautiful single cone #Photoreceptor types in the #retina of #chicken expressing #GFP via my #Intravitreal AAV injections. For more insight, check out my recent publication (www.frontiersin.org/journals/phy...) Great images for #FluorescenceFriday. #birds #AAVs #injections #transduction

Pleased to share an exciting update on our recent publication “AAV-mediated transduction of songbird retina” lnkd.in/eeu_q5aF In two months, it attracted: 1,158 views and 259 downloads. This strong engagement reflects growing interest in #genetictools for #avian models in #vision #neuroscience.

Sharing another beautiful image of a #Gallus gallus ( #chicken ) #retina, stained with various markers, taken by me for #FluorescenceFriday What better way to start a Friday. Retina is a beauty.

Happy to share that my latest research paper titled "AAV-mediated #transduction of #songbird #retina" which was published in the journal "Frontiers in Physiology", in its special issue "Rising Stars in Avian Physiology: 2024", reached a #1000 views in less than 2 months. lnkd.in/e_8yQtKA

One of my recent articles, published in Frontiers in Physiology, has just reached a new impact milestone. It got a 100 downloads in 17 days. Here is the link if you would like to read our study: www.frontiersin.org/article/1549...

Congrats to our researchers around @thebuddhabird93.bsky.social on their latest joint publication: Using a custom-designed injection setup, they identified a #viral #serotype with the capability to successfully induce widespread transduction of the bird retina. doi.org/10.3389/fphy...

I am delighted to present to all my latest research paper titled " #AAV-mediated transduction of #songbird #retina" which has been published in the journal " #Frontiers in #Physiology", in its special issue "Rising Stars in Avian Physiology: 2024". #birds #AAV www.frontiersin.org/journals/phy...

ICARUS BIRDS Where, how, and why do birds die? We are launching the ICARUS BIRDS project to find out! Join us in tracking common birds across Europe to uncover the factors behind their decline. Learn more on our website: www.ab.mpg.de/icarus

Another beauty that I came across during my #2-photon experiments is a Polyaxonal amacrine cell (PAC), a type of #interneuron in the #retina with multiple axon-like processes, said to play a crucial role in visual signal processing and modulation, particularly in the #IPL.

We are happy to introduce our lab members - today: Annika @annikapeter.bsky.social! She studies songbird navigation skills for her PhD & is fascinated by all kinds of birds & enjoys observing their behaviour, both at work & in her free time. Wheatears have a special place in her heart ♥️

2 beautiful cells expressing #GCaMP in #chicken retina via AAV-mediated retinal #transduction. The dendritic fields of these cells are vast, beautiful and ordered, depicting how truly intricate #retina connectivity is. #Avian retina is remarkable. My choice for today's #FluorescenceFriday image.

I have an open position for a postdoc to study neurodevelopmental disorders in the retina using two-photon imaging and electrophysiology!

First attempt at 3D re-constructing the #Horizontalcell from my transduced #chick #retina. Cells can be very dramatic. All you need is a GIF and some Pavarotti notes 🎵 Sample by @neurofishh.bsky.social Great time for some #FluorescenceFriday.

An evening with the #Starlings, with them #murmuring and spending their last few days of wintering in #Brighton. What a mesmerizing sight. #birds #avian

The beautiful #Horizontalcell types in the #retina of #chicken The distinction in the dendrites is amazingly clear, with each dendritic branch contacting a certain #photoreceptor type. #Avian retinal #neurons are truly mesmerizing. Great images for #FluorescenceFriday. #birds #AAVs #injections

A step towards a standardized nomenclature of photoreceptor rods and cone across vertebrates. Do read the pre print for more. www.preprints.org/manuscript/2...

The most beautiful neurons in the brain (fact), and also my villain origin story - #FluorescenceFriday 🧡 biocytin patched and filled Purkinje cell 💚 parvalbumin 💜 AnkG for axon initial segments

This is magnificent

A group of scientists who are experts at spotting fraudulent research outline five essential steps to combat industrialized scientific misconduct https://go.nature.com/4h78aSw

When your #avianretina markers meant for staining, #ONbipolarcells accidentally revealed the #indian flag 🇮🇳. Happy 76th #RepublicDay to all fellow Indians. #immunohistochemistry #UniversityofSussex #Fluorescencefriday

The beauty of viral transfection in #avianretina 🐔 Here is a transfected #amacrinecell and some #photoreceptors in a cross-section of a #chick #retina. Great image for #FluorescenceFriday Sample from @neurofishh.bsky.social Green: Viral transfected retina; Red: DAPI for stratification

Are you PhD student or Postdoc working in the field of Neuroethology? Then apply as a speaker for the Webinar Series 'The Future Of Neuroethology'

Electric fence or radial glia? These cells🟡 are present only during 🧠development in the neocortex and guide immature neurons 🟣to their final position! Microglia/macrophages in 🔵. It was supposed to be a #FluorescenceFriday, now a #SynapticSunday, maybe #MicroscopyMonday, depending where you are! 🤓

Bit gloomy on Christmas morning in Bartley Green Birmingham. However, this Robin brightened things up a bit. 100-400mm + 2X at 5000 ISO just about got a useable shot! #birds #ukbirding #photography #ukwildlife #uknature