🚨 Today we report cryo-EM structures of the Bxb1 integrase complexed with its attB/attP DNA substrates before, during and after recombination. Using these structures, we engineer Bxb1 mutants with improved recombination efficiencies and altered sequence recognition properties. Check it out! ⬇️

If you like transposons... If you you love genome editing... Or if you just like random bird animations, we have the paper for you! We (@kedmonds.bsky.social et al) are happy to share our work turning a songbird retrotransposon into a genome editing tool. 🐣 (1/n)

STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions. Plasmids here www.addgene.org/browse/artic...

So excited to share our new paper out today in @nature.com. We repurposed R2 retrotransposons to create STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions 🧵 www.nature.com/articles/s41...

🚨 Interested in precise, multi-kilobase scale insertions into the genome? Check out our detailed protocol describing PASTE, out in Nature Protocols @natureportfolio.bsky.social ⬇️ www.nature.com/articles/s41...