Nederlandse Entomologische Vereniging (@nev.nl)

Spectral Characterization of the Life Stages and Physiological Responses of Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) Larvae Parasitized by Cotesia flavipes Cameron (Hymenoptera: Braconidae) Hyperspectral proximal sensing was used to characterize the life stages and physiological responses of Diatraea saccharalis and to detect parasitism by Cotesia flavipes. Distinct spectral signatures differentiated eggs, larval instars, pupae, and adults, as well as live, dead, and parasitized larvae. Multivariate analyses (PCA) clearly separated developmental stages and physiological conditions, demonstrating the potential of hyperspectral reflectance as a rapid, non-destructive tool for insect phenotyping and biological control monitoring. https://onlinelibrary.wiley.com/doi/10.1111/eea.70076