Phil Kyriakakis
@breakliquid.bsky.social
📤 2218
📥 3219
📝 745
Senior Research Scientist at Stanford Bioengineering | Taking apart and building biology.🧪
Two preprints up in the same week! This one was from work I finished in 2014, my PhD work! FINALLY. It describes the biochemistry of the yeast glucokinase, which is in the actin family, and forms ATP/ADP-regulated glucose-6-phosphate filaments!
www.biorxiv.org/content/10.6...
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Glk1p is an actin fold metabolic enzyme whose polymerization is sensitive to nucleotide state
The actin fold is present in enzymes ranging from sugar kinases to chaperones. Previous work on the actin fold metabolic enzyme, glucokinase (Glk1p) in S. cerevisiae found it could form filaments in r...
https://www.biorxiv.org/content/10.64898/2026.07.09.731748v1
10 days ago
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OooooopenEvo! What a journey building this
@atinygreencell.bsky.social
, our baby is born! We hope this serves as a nice research tool as well as a teaching tool for biology, electronics and more!
add a skeleton here at some point
12 days ago
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Digital Brain
18 days ago
Peacock spiders are only 4mm long, but their courtship dance rivals any bird of paradise. Australia has over 90 species of these tiny, ultra-colorful performers.
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Is this cool or gross?
www.nature.com/articles/s41...
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Underwater Suit-Wearing Cyborg Insect Capable of Hours-Long Diving and Terra-Aqua Travel - Nature Communications
A wearable diving suit enables terrestrial cyborg cockroaches to maintain respiration and locomotion underwater for up to 3 hours. This biohybrid system facilitates sustained exploration through hybri...
https://www.nature.com/articles/s41467-026-74235-1
21 days ago
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I thought maybe I invented this term, but its already a thing! Its better than PCR, GCR! “Gratitude Chain Reaction uses the psychological ripple effect of appreciation to inspire a cycle of reciprocal kindness”
24 days ago
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Phil Kyriakakis
Sebastian S. Cocioba
about 1 month ago
I made a webpage to try SpliceCraft out directly in your browser in a little sandbox. No download or install needed to try it. Gonna make a tutorial mode to walk people through the features soon. Enjoy!
splicecraft.bio
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SpliceCraft — the in-terminal plasmid workbench
Design, clone & mutate plasmids in a mouse-friendly terminal workbench. Everything runs on your own computer — no cloud, no account.
https://splicecraft.bio/
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Stacey Ogden 🦔
about 2 months ago
Make some noise about this 🧪
www.npr.org/2026/06/03/n...
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President Trump seeks control of science funding
The White House Office of Management and Budget is moving to take more control of billions of dollars in federal grants. Critics say the proposed change would jeopardize the integrity of U.S. science.
https://www.npr.org/2026/06/03/nx-s1-5844678/trump-science-funding-omb-budget-office-rule-change
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WHY?
about 2 months ago
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Phil Kyriakakis
Sebastian S. Cocioba
about 2 months ago
SPLICECRAFT v1.0 IS LIVE!!!!!!!!!!!! Open your terminal and type in "pipx install splicecraft" if you want to try it out, then spam "splicecraft update" often as I push updates frequently. A labor of love for the community I adore. Enjoy! 💚
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Exam question idea: give a question with AI answering it. The student needs to explain what is wrong with the AI answering.
2 months ago
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Night market in Santa Clara this weekend!!!
www.santaclaraca.gov/recreation-c...
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https://www.santaclaraca.gov/recreation-community/events/super-bowl-lx-and-fifa-world-cup-2026/celebrate-santa-clara-2026-community-events/night-markets
3 months ago
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Evolution of my print. Printing TPU 85a is way harder than I expected.
3 months ago
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I accidentally made a better stirring system
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4 months ago
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Much needed good news
www.canarymedia.com/articles/cle...
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Suddenly, the US manufactures a ton of grid batteries
Energy storage is surging on the U.S. grid — and now the country has more than enough battery-making factories to meet that booming demand.
https://www.canarymedia.com/articles/clean-energy-manufacturing/us-capacity-storage-cell-factories
4 months ago
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Either I like to eat scorpions or I don’t…
4 months ago
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Claude is way better anyway
add a skeleton here at some point
5 months ago
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Mass surveillance and killer robots are not for me.
5 months ago
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My new cup holders, which change color when heated.
5 months ago
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Phil Kyriakakis
Andy Rivkin
5 months ago
🔭🛰️🧪
add a skeleton here at some point
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Princess Vimentin PhD | Cancer Biologist
5 months ago
Exciting! Stanford researchers have developed a UNIVERSAL INTRANASAL vaccine. A study in mouse models shows that vaccinated mice were protected against Covid & other coronaviruses. Still a long way to humans. This study was
#NIH
funded. A potential game changer. 🧪
med.stanford.edu/news/all-new...
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One vaccine may provide broad protection against many respiratory infections and allergens
Stanford Medicine researchers and their colleagues invented a new vaccine that protects mice from respiratory viruses, bacteria and allergens — the closest yet to a universal vaccine.
https://med.stanford.edu/news/all-news/2026/02/universal-vaccine.html
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Wim Meijer
5 months ago
The system acts as near-permanent archival storage for backup of critical data. It stores 4.8 terabytes, the equivalent of around 2 million printed books Data survives for 10,000 years at a temperature of 290ºC and much longer at room temperature. 🧪🦠
www.nature.com/articles/d41...
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Microsoft team creates 'revolutionary' data storage system that lasts for millennia
Researchers use mini plasma explosions to encode the equivalent of two million books into a coaster-sized device. The method could preserve research data for millennia with minimal storage costs.
https://www.nature.com/articles/d41586-026-00502-2
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Phil Kyriakakis
Tapani Hopkins
5 months ago
I suspect one reason science social media has done reasonably well post-Twitter is that it didn't get (too badly) scattered. There's a viable population on both Bluesky and Mastodon. Here's one (opt-in) way of of connecting those two populations to make sciencesky even bigger. 🧪
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Nigel Goldenfeld
5 months ago
🧪⚛️ Ever wondered what would happen if you thrashed around in a water tank, creating a blob of turbulence, and then watched it spread and gradually dissipate energy? So did we. But we did the experiment, now out in PNAS and on the cover! It only took us 7 years ...
www.pnas.org/doi/10.1073/...
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Why is Windows do this every time my computer updates?
5 months ago
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Phil Kyriakakis
Tim O'Connell
5 months ago
Okay, but for
#BlueSky
to gain a fraction of the value in networking that we once enjoyed over at
#Twitter
, we all need to be reposting pretty much everything we see. Scientists no Longer Find Twitter Professionally Useful, and have Switched to Bluesky url:
academic.oup.com/icb/article-...
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Scientists no Longer Find Twitter Professionally Useful, and have Switched to Bluesky
Synopsis. Social media has become widely used by the scientific community for a variety of professional uses, including networking and public outreach. For
https://academic.oup.com/icb/article-abstract/65/3/538/8196180
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🤞
add a skeleton here at some point
5 months ago
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Another cool pipette rack pattern!
6 months ago
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Phil Kyriakakis
Colette Delawalla, PhD
6 months ago
Wow. This is devastating.
www.science.org/content/arti...
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U.S. government has lost more than 10,000 STEM Ph.D.s since Trump took office
A Science analysis reveals how many were fired, retired, or quit across 14 agencies
https://www.science.org/content/article/u-s-government-has-lost-more-10-000-stem-ph-d-s-trump-took-office
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Another 3d printed pipette rack
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6 months ago
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My new pipette holder
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6 months ago
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This figure really grabs your attention!
6 months ago
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Is there an easy way to get saw RNA-seq data and turn it into a table of differentially expressed genes? Like, without installing 12 programs and taking a bioinformatics course?
6 months ago
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I hope their antibodies are better than their legos. The design is very flimsy, but I enjoyed the challenge.
6 months ago
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Taaaaalllllll
7 months ago
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Phil Kyriakakis
BWJones
7 months ago
Surprise mother$#@%er! Q: What does this mean for neural systems to understand them? A: We absolutely need the wiring diagram. All synapses, chemical and electrical.
#Connectomics
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They win for best use of parafilm:
www.nature.com/articles/s41...
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Fluorine-free binder-based dry thick electrodes with Parafilm® M toward sustainable and efficient battery manufacturing - Nature Communications
Fluorine-containing binders in battery dry electrode processing raise environmental concerns regarding restrictions on per- and polyfluoroalkyl substances. Here, authors show that a fluorine-free bind...
https://www.nature.com/articles/s41467-025-66082-3
7 months ago
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👀
add a skeleton here at some point
7 months ago
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How to write an R01 type grant that isn’t “too high risk”, but is still exciting, novel?
7 months ago
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Phil Kyriakakis
Mark Peifer (He, him)
7 months ago
This is VERY cool! 🧪
add a skeleton here at some point
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Son got sick (severe cold) in Taiwan, no insurance. Saw a doctor in 10min, $15 including the acetaminophen and ibuprofen the doctor gave us. How much would that be in the USA?
7 months ago
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More squirrel.
7 months ago
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This website was meant for scientist runners! They literally cut the shoes in 1/2 and do all sorts of testing on them!
runrepeat.com/saucony-ride...
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Cut in half: Saucony Ride 17 Review (2024)
Saucony Ride 17 review: The Saucony Ride 17 is more than just a minor update—it's a significant overhaul, especially with the all-new midsole that enhances energy return and responsiveness. In our lab...
https://runrepeat.com/saucony-ride-17
8 months ago
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Phil Kyriakakis
Oded Rechavi
8 months ago
PI sharing with his student a competitor's ERC that he's reviewing
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Wow, this is really cool!
www.nature.com/articles/s41...
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All-optical visualization of specific molecules in the ultrastructural context of brain tissue - Nature Biotechnology
Pan-expansion microscopy of tissue combines staining of proteins and lipids with immunolabeling.
https://www.nature.com/articles/s41587-025-02905-4
8 months ago
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Update, I grabbed 10 colonies and combined into one
@plasmidsaurus.bsky.social
reaction. All of them were too small and most were the same, the TE inserted in the same place. So screening colonies won't likely work.
add a skeleton here at some point
8 months ago
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Phil Kyriakakis
Jaeseung Hahn
8 months ago
Boolean logic-gated protein cargo release!
@coledeforest.bsky.social
's group previously used the molecular topology with proteases as inputs to release fluorescent proteins from hydrogels, and they now extended the platform for other protein cargoes! 🧬
onlinelibrary.wiley.com/doi/abs/10.1...
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Has anyone had this show up in their plasmid, and how to prevent it? "E. Coli IS10L Transposon"
8 months ago
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AI can mess things up real fast! 😭
8 months ago
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I tried to "squeeze gel purify" DNA, sent it for sequencing, and it worked! ~Protocol: 1) cut out band 2) poke hold in small tube, put gel in it, put in big tube 3) Spin for 2-3 seconds 4) sequence the liquid in the bottom tube This may not always work, but cool!
@plasmidsaurus.bsky.social
9 months ago
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iGEM is not your typical scientific conference!
@igemcommunity.bsky.social
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9 months ago
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