And it's out! Our paper on the optimization of smFISH to quantify cytokine mRNAs in T cells is published in EMBO Journal. Using this protocol, we gathered interesting insights on cytokine mRNAs co-expression and localization in these teeny tiny human cells. www.embopress.org/doi/full/10....

#spatialbiology #RNAbiology www.nature.com/articles/s41...

It's out! Beautiful work led by Loreto Oyarte Gálvez & @cbisot.bsky.social! Do you want to learn more about how Mycorrhizal fungi extraordinary networks form and function? Read the open-access publication! Link below 👇

I am giving a lecture at the EMBO practical course: "FISHing for RNAs: classical to single molecule approaches" Check out this hands-on EMBO course!

Excited to share @domenicnarducci.bsky.social 's paper now out in Mol Cell published back-to-back with the paper from @dewitlab.bsky.social‬ - despite lots of prior work, we find that ZNF143 has no looping function www.sciencedirect.com/science/arti... www.sciencedirect.com/science/arti...

MDPI & Frontiers journals blacklisted in Finland, I wonder if this will indeed spread further? Never considered any of these journals for publication, but MDPI surely send funny emails... Maybe I will introduce myself like this 👇 from now on 😂.

"We report that numerous “miRNAs” previously reported in EMT and cancer contexts are not incorporated into RISC and are not capable of endogenously silencing target genes, despite the fact that hundreds of publications in the cancer field describe their roles" www.biorxiv.org/content/10.1...

Not easy to describe the emotions of seeing our work published in @cellpress.bsky.social ! We explored how novel isoforms emerge during evolution through the exonization of Transposable Elements. Exonized TEs modify the functions and structures of host proteins. www.cell.com/cell/fulltex...

📢We're recruiting📢 Our lab at the University of Lausanne is looking for a postdoctoral associate to study #nucleotide metabolism in physiology and cancer in vivo! Please share #uridine #cancermetabolism #immunometabolism @unil.bsky.social 🔗(PDF): www.jourdainlab.org/wp-content/u...

Excited to share our new work on the earliest steps of Pol-III transcription: We provide structural and kinetic insights into tRNA promoter engagement by yeast transcription factor TFIIIC ! Cool collaboration with the Müller and Eustermann Labs @embl.org ! academic.oup.com/nar/advance-...

🧪 Your eyes may deceive you. A study of 3,387 life-science papers from 2023 found nearly 1/3 of bar charts distorted data, often by not starting the y-axis at zero or misusing log scales.

🎉Excited to share our new preprint on Biorxiv 🥳 We uncover how RNA Polymerase III (Pol3) dynamically regulates tRNA transcription during heat shock (HS) stress! So what did we find? 🧵 www.biorxiv.org/content/10.1...

Metabolism and mRNA translation: a nexus of cancer plasticity www.cell.com/trends/cell-...

Paired CRISPR screens to map gene regulation in cis and trans https://www.biorxiv.org/content/10.1101/2024.11.27.625752v1

Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes https://www.biorxiv.org/content/10.1101/2024.11.26.625372v1

Very cool - snoRNA73 acts as a bridge between mRNAs and 7SL (i.e. the SRP ncRNA) to facilitate the synthesis of the encoded secreted protein.

A Versatile Anti-CRISPR Platform for Opto- and Chemogenetic Control of CRISPR-Cas9 and Cas12 across a Wide Range of Orthologs https://www.biorxiv.org/content/10.1101/2024.11.25.625186v1

Did you know that at our conference, we record our Keynote Lectures and make them freely available? You can find them all in our Youtube page! To get started, check out the 2023 IUBMB Jubilee Lecture, given by Prof. Rachel Green.

Post a non-religious photo you think of as holy. Not necessarily holy, just cool contrast between "then and now" in ribosome assembly landscape understanding. Nierhaus map from the 80s / AV Broeck & Sebastian Clinge in Science 2023.

This 👇 is happening. Change is coming!

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 by Ueli Laemmli! The paper which introduced SDS-page to the world!!! 💯

💯 February 14th 1969, RNA polymerases' day. The pioneering experiment that laid the foundation for the eukaryotic transcription field by Robert Roeder.

Post a non-religious photo you think of as holy

Post a non-religious photo you think of as holy.

Reconciling competing models on the roles of condensates and soluble complexes in transcription factor function https://www.biorxiv.org/content/10.1101/2024.11.21.624739v1

IDH-mutant gliomas evolve from slow-cycling OPC-like cells to proliferative NPC-like cells through chromatin reprogramming and genetic alterations. This transition reveals key mechanisms of tumor progression. www.nature.com/articles/s43...

How long does an mRNA stay in the nucleus? How long does it stay in the cytoplasm? In an amazing collaboration with @landthalerm.bsky.social, we used metabolic labeling, cell fractionation and mathematical modeling to quantify mRNA flow through the cell. Finally out in MSB: doi.org/10.1038/s443...

New on BioRxiv: We report a new, endogenous molecular clock in unmodified human cells and tissues. It is ticking away right now in almost every cell in your body. Paper here: www.biorxiv.org/content/10.1... 1/

This is a very cool paper that I will add to my Mol Bio course. Specific tRNA recruitment of mRNA degradation complex to ribosome through the P site tRNA interactions with CCR4-NOT complex 1/ www.science.org/doi/10.1126/...

In collaboration with Reuben Saunders, @jswlab.bsky.social, and Xiaowei Zhuang, we are very excited to release Perturb-Multi: a platform for pooled multimodal genetic screens in intact mammalian tissue. Check it out! www.biorxiv.org/content/10.1...

Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling go.nature.com/4hXER5O

Single-molecule states link transcription factor binding to gene expression www.nature.com/articles/s41...

New preprint on our method aa-tRNA-seq: chemically capturing aminoacylated tRNA molecules & pulling them through nanopores. It’s been a blast working with my collaborator Aleks Radakovic to take this from idea to promising pilot data to some looking exciting biology to chase down in future work.

Hello Bluesky community! What a better way to introduce myself and my research team than post our lab’s first paper freshly off press 🥳🍾 We reveal how tubulins regulate the stability of their mRNA via reversible sequestration of TTC5. Bravo A. Batiuk and all coauthors 👏🏻🥂 doi.org/10.1038/s414...

Our paper on a centromere-enriched retroelement is out! link.springer.com/article/10.1... Congratulations to all the authors! Comments and reposts welcome and appreciated 🧬🔬

Structural basis of tRNA-like small RNA generated from MALAT1 and NEAT1 www.nature.com/articles/s41... www.nature.com/articles/s41...

Don't miss our (updated) work on the developmental regulation of plant nuclear tRNA genes on Bluesky! #RNAsky #TransferRNA #Epigenetic #Chromatin #Tandemrepeat #Cluster #PlantTissue #TissularIdentity #Root #ProteinBiogenesis #Proteostasis #Glycoprotein www.biorxiv.org/content/10.1...

Download our database of 313 different postdoctoral fellowships. For each entry, we provide link to funder, description, amount, deadline, and eligibility criteria (e.g. citizenship). Download our database here: research.jhu.edu/rdt/funding-...

Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm https://www.biorxiv.org/content/10.1101/2024.11.14.623620v1

Happy to share our new paper with Goran Kokic and the Svejstrup lab in Cell! We show that STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair. Great teamwork by Diana, Marta and Paula! www.cell.com/cell/fulltex...

In September the world lost one of the great biologists of our time: Joe Gall. He bridged the transition from the histology era of cell biology to our molecular present, contributing a remarkable set of insights in his 9 decades 1/n 🧪 rupress.org/jcb/article-...

(1/8) 🚀 Excited to share our findings on a large-scale ChIP-seq assay for 166 previously uncharacterized human transcription factors (TFs) and their roles in both regulatory regions, and more strikingly, the “dark matter” genome. 🌌 doi.org/10.1101/2024....

New preprint 📣📣 Together with Monika Wolker's lab @sanquin Amsterdam, we optimized a smFISH protocol for T cells. We measured cytokines IFNG/TNF expression and localization during T cell activation and optimized an imaging pipeline for quantification. www.biorxiv.org/content/10.1...