🤔 #AskZebrafish: Would anyone within the EU have a mutant ybx1 line swimming around and willing to share? Any leads would be appreciated.

🤔 #AskZebrafish: Looking for krt18a reporter fish in the UK or if anyone has a ready to inject construct for making one and are willing to share, that would be much appreciated!

🤔 #AskZebrafish: Looking for Prom1a and/or Prom1b mutant lines and/or reporter lines that someone would be willing to share? Preferably in the USA.

🤔 Query from Slack: Looking for 5' myl7/cmlc2 promoter vector and someone willing to share it? Preferably in the US 🐟🫀 #AskZebrafish

Another query from Slack: Looking for ccdc40 mutant #zebrafish (aka locke). Preferably in the US. Any leads would be much appreciated! #AskZebrafish

Query from Slack: Does anyone have good recommendations for antibodies that label mitochondrial DNA encoded proteins and work well in #zebrafish? #AskZebrafish

Anonymous query from Slack: Does anyone have the TgBAC(foxp3a:TagCFP-NTR)vcc5 line swimming around, preferably in the US? Thanks in advance! #AskZebrafish 🤔

Still on the hunt for fish lines 🙏

Still searching for leads! 🙏

Head-scratcher from Slack: Does anyone know if it is possible to tell (by eye/microscope) if the developing oocytes in a zebrafish ovary are at the pre-, during, or post-vitellogenesis stage? 🤔 #AskZebrafish

Looking for anyone w/ experience in smRNA analysis who can provide someone with information or guidance? They already have their sequences, but can't find a clear methodology/protocol to help them analyze it. The sequences were obtained from NOVOGENE's Small RNA Sequencing service. 🤔 #AskZebrafish

Any leads would be appreciated 🙏

Any leads would be appreciated! Reposts welcome 🙏

Still searching for leads 👀

Still looking for leads 🕵️

Query from our Slack Tankspace: Does anyone in Europe have the Tg(mpx: mcherry ) line from the Huttenlocher Lab or similar and would be willing to share? #AskZebrafish

Still looking for leads 🕵️

Re-upping

Another query from Slack: Does anyone (preferably in the US) have the Tg(GFAP:iGluSnFR) line and be willing to send them to another #zebrafish lab? 🤔 #AskZebrafish

🤔 #AskZebrafish

Still looking for guide sequences! Any leads and/or help appreciated!

🤔 #AskZebrafish

Query from Slack: Does anyone have scn8aa and scn8ab #zebrafish mutants and would be willing to share some embryos? (Preferably in Europe, if possible) 🔎 #AskZebrafish

🤔 #AskZebrafish

Re-upping 🙏

🤔 #AskZebrafish

Re-upping

Re-upping

Query from Slack tankspace: “Looking for transgenic lines [preferably in Europe] marking mesoderm/mesendoderm and ectoderm, with expression before 6hpf. Thinking of mezzo and sox3, if anyone has these? Any other suggestions would also be great.” #AskZebrafish

#AskZebrafish from Slack: "Does anyone have a vector they recommend for poisonous inserts? We are using a bacterial strain with high expression of LacI to prevent expression from our insert and the bacteria still mutate the construct - we assume to render it a non-functional channel."

Re-upping

If this is your facility, please reach out!

Still searching for leads!

Query from Slack: Looking for a lab with the TgBAC (gata5:GFP)pd25 line swimming around in the USA and would be willing to share? 🤔 #AskZebrafish

If anyone has these fish or have any info about who might, please reach out 🙏

Re-upping

Re-upping

Anonymous #AskZebrafish: What is the best practice for generating a stable CRISPR/Cas9 knockout (i.e. to avoid genetic compensation)? Guides flanking either side of the gene? Around the promoter sequence? Use of an ssODN? Any advice would be thoroughly appreciated.

Query from Slack: Does anyone in the US have a dominant negative integrin (β1) construct under a heat-shock promoter that they could share? Any leads would be thoroughly appreciated! #AskZebrafish

Re-upping 🙏

Query from Slack: Wondering if anyone has already tested the new HCR v4.0 version (HCR™ Gold RNA-FISH) and if it worked well in fish, in comparison with v3? 🤔 #AskZebrafish

@askzebrafish.bsky.social can a #zebrafish kidney person explain to me how the mesonephros gets all the wait to the dorsal part of the animal's head when it starts so ventral in the trunk? I can't find a good picture or diagram. Thanks much

🤔 #AskZebrafish

🤔

Anonymous question from Slack: What RO machine brands do folks use (and like) for supplying aquatics housing systems? (Not necessarily for fish) 🤔 #AskZebrafish

Another query from Slack: Does anyone in the Netherlands (or a neighboring country) happen to have the ubi:creERT2 line and would be willing to share? 🤔 #AskZebrafish