Claire Leclech (@claireleclech.bsky.social)

My last piece of work is now on bioRxiv 🤩 We describe an original pattern of collective movement in unconfined monolayers of endothelial cells on microgrooved substrates. Go and check it out! biorxiv.org/content/10.110…

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Topography-induced large-scale anti-parallel collective migration in vascular endothelium Collective migration of vascular endothelial cells is central for embryonic development, angiogenesis, and wound closure. Although physical confinement of cell assemblies has been shown to elicit specific patterns of collective movement in various cell types, endothelial migration in vivo often occurs without confinement. Here we show that unconfined endothelial cell monolayers on microgrooved substrates that mimic the anisotropic organization of the extracellular matrix exhibit a new type of collective movement that takes the form of a periodic pattern of anti-parallel cell streams. We further establish that the development of these streams requires intact cell-cell junctions and that stream sizes are particularly sensitive to groove depth. Finally, we show that modeling the endothelial cell sheet as an active fluid with the microgrooves acting as constraints on cell orientation predicts the occurrence of the periodic anti-parallel cell streams as well as their lengths and widths. We posit that in unconfined cell assemblies, physical factors that constrain or bias cellular orientation such as anisotropic extracellular matrix cues or directed flow-derived shear forces dictate the pattern of collective cell movement. ### Competing Interest Statement The authors have declared no competing interest. https://www.biorxiv.org/content/10.1101/2021.07.05.451106v1