If anyone was curious. We succeeded, but it wasn’t easy. Reviewers were insistent on western blots. Eventually we convinced the editors otherwise using peptide level quant info to show decent sequence coverage and a biochemical/biophysical rational for why not all the peptides had the same quant.

How are people handling the “validate your protein change by western blot” reviewer request? We already have proteomics data showing protein KD, qPCR showing transcript KD, and a dramatic functional response. I am leaning towards holding my ground not to do western. It is just redundant busy work

After a 15 year hiatus, I’ve got a new paper with the Coon lab. Led by @liaserrano.bsky.social, we kicked the tires on the Orbitrap Astral for PPI studies. Since we run thousands of APMS samples/yr we focused on fast analysis (7 min run, >200 samples in 29hr). pubmed.ncbi.nlm.nih.gov/40025722/