My main PhD work @monteirolab.bsky.social is now in @natecoevo.nature.com! We found a Hox gene promoter that helps butterflies🦋adjust their wing eyespots in response to seasonal temperatures🍃🍂, shedding light on the evolutionary origin of phenotypic plasticity. 1/9 www.nature.com/articles/s41...

Happy to share that the preprint of our updated spatial mRNA multiplexing using RAMFISH (v2.0) is out... www.biorxiv.org/content/10.1... Special thanks to all the collaborators for their efforts.

Excited to share that our Geneplexer and Multiplexer systems can now achieve spatial mRNA signal detection within 4.5 hrs, including hybridization, secondary amplification, and washes. Image: A z-slice (~5 µm) of a whole 14 dpf WT zf larva stained against markers nrp1a, gad2, and neuroD.

Happy to collaborate with Ajay Mathuru's team at NUS. "Role of chrna5 in multi-substance preference and phenotypes comorbid with the development of substance dependence." Congratulations to the co-first authors, Tanisha Goel and Joshua Raine. www.biorxiv.org/content/10.1...

Glad to share the final piece of my PhD thesis. We discovered a novel Hox gene promotor that might have facilitated the evolution of phenotypic plasticity across a butterfly subfamily. www.biorxiv.org/content/10.1...

Happy to share that our recent Bicyclus larval brain multiplex-FISH paper has been featured on the cover of Methods and Protocols. www.mdpi.com/2409-9279/8/2

Preprint! Minos-mediated transgenesis in the pantry moth www.biorxiv.org/content/10.1... By @donyaniyaz.bsky.social

Sharing a user friendly online app (beta phase) to design probes for HCR3.0 tdblab.github.io/hcrprobedesi...

Happy to share our latest 3D whole-mount multiplex-RNA brain scans of the larval brains of Bicyclus anynana at single-cell resolution... We mapped multiple targets at single-cell resolution and made the raw data open source for anyone who would like to analyse it. www.mdpi.com/2409-9279/8/...

New preprint by butterfly wizard @lucalivraghi.bsky.social www.biorxiv.org/content/10.1... We mapped optix (again?!) as the switch gene of a natural polymorphism, this time controlling silver patches of a mountain butterfly. Gorgeous RNAi validation, evidence of selective sweeps, introgression

Our new manuscript describing gene multiplexing (using RAM-FISH) is finally out... We profiled multiple known and novel mRNA candidates during different developmental stages of Bicyclus and zebrafish using the easy-to-use HCR-based protocol that most labs can adapt. www.biorxiv.org/content/10.1...

Happy to be a part of the work by @tianshenbio.bsky.social published @science.org Excellent work and many more to come🎉

Our miRNA story is now in @science.org ! We found a microRNA, not a protein, that finally solved a long-standing evolutionary mystery of wing coloration in butterflies and moths. (1/n) www.science.org/doi/10.1126/...