Louise Johnson
@drlouisejohnson.bsky.social
📤 156
📥 130
📝 16
Immunologist/cell biologist, also a fan of beautiful places. Warning: may post cat pics
reposted by
Louise Johnson
ProImmune
4 days ago
This
#FluorescenceFriday
we use our Ankyron CJ30442 (cyan) to identify the lymphatic marker LYVE1 in skin, showing the extensive lymphatic network through to the individual oak-leaf-shaped endothelial cell on the Mica
#microscope
@leicamicrosystems.bsky.social
. Learn more at
proimmune.com/ankyron
.
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reposted by
Louise Johnson
ProImmune
2 months ago
This
#FluorescenceFriday
we are shining a light on lymphatics in skin using Ankyron® clone DE40213, which binds Podoplanin (cyan). Image captured with our
@discoverecho.bsky.social
#microscope
. Learn more about our recombinant antibody-replacements at
proimmune.com/ankyron
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reposted by
Louise Johnson
ProImmune
4 months ago
This
#FluorescenceFriday
we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant
@discoverecho.bsky.social
#microscope
.
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reposted by
Louise Johnson
ProImmune
5 months ago
This
#FluorescenceFriday
we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely
@discoverecho.bsky.social
#microscope
. Learn more:
proimmune.com/ankyron
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Happy
#Caturday
! Princess Fifi Trixibelle Murder Mittens (first of her name) is enjoying the weekend
#cats
#adoptdontshop
#pond
8 months ago
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reposted by
Louise Johnson
ProImmune
10 months ago
Fascinating insights this
#FluorescenceFriday
, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on
@discoverecho.bsky.social
#microscope
. Find out more about our
#animal-free
Ankyrons™ at
www.proimmune.com/ankyron
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I wish “smell-o-vision” was a thing, as these
#lilyofthevalley
smell incredible.
#Spring
#April
#gardenpond
#nativeplant
#uk
10 months ago
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reposted by
Louise Johnson
ProImmune
10 months ago
Exquisite filamentous patterns for
#FluorescenceFriday
thanks to Ankyron™ AU41488 specific to CD29 cell surface receptor β1 integrin (red) in a dendritic cell, with nucleus counterstained with DAPI (blue), on our lovely
@discoverecho.bsky.social
Echo Revolve
#microscope
.
#immunology
#animalfree
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Looking through the leper’s squint into a church that is approaching its 1000th birthday
#uk
#history
#england
10 months ago
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Happy
#Caturday
! Not sure how long my Easter decorations will stay in place though
10 months ago
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reposted by
Louise Johnson
ProImmune
11 months ago
#MicroscopyMonday
! IHC with an insulin-specific
#animalfree
Ankyron AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) usingÂ
#Leica
autostainer and
@discoverecho.bsky.social
#microscope
in colour camera upright configuration.
proimmune.com\ankyron
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reposted by
Louise Johnson
ProImmune
11 months ago
This
#FluorescenceFriday
we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on
@discoverecho.bsky.social
microscope. Look out for our other organelle-specific
#animalfree
Ankyrons™ at
proimmune.com\ankyron
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I disturbed another occupant of the Oxford Science Park but they were illegally fishing - goldfish saved!
11 months ago
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reposted by
Louise Johnson
ProImmune
11 months ago
Shining a light on Endoglin (aka CD105) for
#FluorescenceFriday
, shown in red using
#animalfree
Ankyron™ clone AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC with crucial roles in angiogenesis & TGFβ signaling. On our
@discoverecho.bsky.social
#microscope
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reposted by
Louise Johnson
ProImmune
11 months ago
This dendritic cell is ready for
#FluorescenceFriday
! Here, we used our interferon-α8-specific
#animalfree
Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on
@discoverecho.bsky.social
#microscope
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social
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reposted by
Louise Johnson
ProImmune
11 months ago
#FluorescenceFriday
insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our
#animalfree
Ankyron™ - nuclei counterstained with DAPI (blue) on
@discoverecho.bsky.social
#microscope
#bioscience
#immunology
#animalfreeresearch
#cytoplasm
@afruk.bsky.social
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reposted by
Louise Johnson
ProImmune
11 months ago
Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3
#COVID19
spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay.
doi.org/10.1208/s122...
#BLI
#immunology
#animalfree
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reposted by
Louise Johnson
British Society for Immunology
11 months ago
🚨 Last chance to register for BSI Oxford and
@immunologyoxford.bsky.social
's Oxford Immunology Symposium!
#OIS25
📅 Date: 24 March 2025 📍 Location: Oxford Mathematical Institute 🎤 Featuring talks on adaptive & innate immunity and interdisciplinary insights Register NOW:
t.co/tlXp9dddqo
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reposted by
Louise Johnson
ProImmune
12 months ago
It's
#FluorescenceFriday
! Here's a lovely clear image using
#animalfree
Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) on
@discoverecho.bsky.social
#microscope
Find out more
www.proimmune.com/ankyron
#immunology
#3Rs
@afruk.bsky.social
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reposted by
Louise Johnson
ProImmune
12 months ago
Is
#WesternBlotWednesday
a thing?! Why not celebrate those perfect western blots? Here's our
#animalfree
Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!
#3Rs
#westernblot
@afruk.bsky.social
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I love being part of this project and product line. Also, continually impressed at the sharpness of the images we achieve on our (epifluorescence) Echo Revolve microscope through the digital haze reduction software
add a skeleton here at some point
12 months ago
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reposted by
Louise Johnson
ProImmune
12 months ago
Spectacular cell structure
#FluorescenceFriday
, with an
#animalfree
Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) on our Echo Revolve
#microscope
#bioscience
#immunology
#3Rs
@AFR_UK
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For
#WindowsWednesday
a view of Stourhead
#nationaltrust
from a room not usually open to the public. The guides were amazing and really brought it to life
12 months ago
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reposted by
Louise Johnson
ProImmune
about 1 year ago
Delicate red dendrites for
#FluorescenceFriday
- here our
#animalfree
Ankyron binds Tumor Necrosis Factor Receptor Superfamily member 1B (TNFR2, in red) - Nuclei counterstained with DAPI (blue) on @Discover_Echo microscope
#immunology
#3Rs
#biomedical
#research
@AFR_UK
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Not sure if it is a thing on this platform but happy
#caturday
to all who celebrate (also please check out the amaryllis that I have managed to not kill yet)
about 1 year ago
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reposted by
Louise Johnson
ProImmune
about 1 year ago
It's February
#FluorescenceFriday
! Here's a beautiful image of
#animalfree
Ankyron™ against proliferation marker Ki67 (red) in dividing HeLa cells counterstained with phalloidin (showing actin cytoskeleton in green) @Discover_Echo
#immunology
#cells
#biomedical
#3Rs
@AFR_UK
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reposted by
Louise Johnson
ProImmune
about 1 year ago
It's
#FluorescenceFriday
! We used our
#animalfree
antibody replacement Ankyron™ (only 15kDa) specifically binding CD31 (PECAM1) fluorescently tagged & small enough to get into tight junctions of endothelial cells using Echo Revolve
#microscope
@Discover_Echo
#immunology
#3Rs
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reposted by
Louise Johnson
ProImmune
about 1 year ago
Banish January blues this
#FluorescenceFriday
and look into the lysosomes of Dendritic cells using our animal-free antibody replacement Ankyron™ binding LAMP3 (red), with nuclei counterstained with DAPI (blue). Imaging carried out with our @Discover_Echo
#microscope
.
www.proimmune.com/ankyron/
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reposted by
Louise Johnson
Clive McKimmie
about 1 year ago
See this super cool seminar by Tineke Contort (
@pasteur.fr
Cambodia) that I am helping to co-chair with Tim Morrison (Colorado) THIS THURSDAY by zoom. All welcome!
add a skeleton here at some point
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Is there anybody here?! Looking for a new social media platform where people share ideas and cool pics in a non-aggressive manner. Here’s a pic I took last weekend, going back to the village in which I grew up
over 1 year ago
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