Yukti Dhingra (@yuktidhingra.bsky.social)

Happy to share the first paper from my postdoc in the Darst and Campbell labs! 🧵 www.pnas.org/doi/full/10.... We used cryo-EM and allosteric inhibitors as mechanistic probes to visualize conformational landscapes of E. coli and M. tuberculosis RNA polymerases during nucleotide addition. 1/4

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RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle | PNAS The nucleotide addition cycle (NAC) of multisubunit DNA-dependent RNA polymerases (RNAPs) involves coordinated conformational changes in conserved ... https://www.pnas.org/doi/full/10.1073/pnas.2609228123

11 days ago