pH gradients are central to physiology, from vesicle acidification to the acidic tumor microenvironment. But how do we program proteins to respond to pH? In our new preprint biorxiv.org/content/10.110…, we developed computational methods to rationally design pH-sensitive binders. 🧵

New research in Science represents a notable step forward in designing enzymes from scratch. With a new approach, researchers designed an enzyme that uses a covalent intermediate to catalyze a two-step reaction, analogous to what many proteases do when breaking apart proteins. scim.ag/41kZBOI