The biggest discount your lab will ever get? (a thread 🔬💰👇)

🧬 DNA found no candidate resistance mechanism in 51% of resistant patients. 📃 mRNA explained only ~40% of protein-abundance variation. 🧱 Protein abundance explained only ~10% of enzyme-activity variation. One omic layer is not the whole truth. Omics need YOUR judgment. — Wildtype One

Don't always trust expiry labels on lab reagents (at least if you're in a rush) Here's proof from a wild (and true) story 👇 (1/5)

It uses the same samples as Western blots. But instead of taking 2 days to reveal 2 proteins... Kinome profiling reveals all 400+ major signaling kinases ...in two hours. (1/5)

Biologists lose some of their work's value when they chase aesthetics: - SEM instead of SD - Dynamite plots everywhere - Not showing sample and effect sizes The biology is in the nuance. 🧬 Catch yourself chasing aesthetics. Show the variability.

Your work isn’t wrong. It might just be early. 🧫

The last year of the PhD/postdoc is NOT "just another year." It is when everything concludes. Like the last episode of a series. (a thread)

According to 2025 data, you can cut 60% of your flow failures with 4 fixes only (see thread 👇)

Scan your manuscript—fraud can be unintentional (a thread)

Stop making “clean” figures that hide your data. Bar charts compress ❌ SEM hides variability ❌ One chart ≠ every experiment ❌ Good figures show: • all points (small n) • distribution (large n) • direction (paired data) If your figure looks clean but hides truth, it’s not good science. 📊

13 cases where your experiments disagree & what it means (a thread 👇)

Before you start your week tomorrow, remember... 🥼👇

Contamination rarely looks like contamination Here are unusual signs we often ignore 🚩👇 (a thread)

Never buy an antibody because of: - Brand ❌ - Price ❌ - Number of citations ❌ Only buy antibodies if: It has been validated for your exact application and species ✅ When buying antibodies, validation is king. It saves you time, money, and failed experiments.

To all hard-working researchers: Working long hours & weekends does NOT prove that you're serious. No one cares how long you work. Exhausted work = less depth = worse judgment We're paid to use our thinking brains. Brain performance requires recharging. — Wildtype One 🧬

The fastest guide to fix Western blots—8 failures in 30 second Save for emergencies 🚨 (A thread👇)

Stop using SEM because it’s cleaner than SD (see thread)

Western blots are great for focused questions. But are not discovery tools. Too many failure modes. Only semi-quantitative. Technically fragile. Use high-throughput methods for discovery. Use western blots to validate. Don't invert the sequence.

You can remove outliers without feeling guilty—under one condition only: If it's a "T-case." What's a T-case? 🤔 Read today's Wildtype Weekly to find out (in 3 minutes or less 👇 )

Imagine Einstein had an hour to fix your experiment 👴🏻 🧬 (a thread)

If your experiments worked but your figures still feel fragile, it’s often not the biology—it’s the analysis. This webinar walks through the 5 most common statistics mistakes biologists make + how to avoid them No coding. No equations. Just clarity. 👉 Register for the webinar here: wildtypeone.com

Irreproducibility undermines the biggest research breakthroughs And we hear more false data today than ever Below are 7 Method-section phrases that can kill doubt and have people trust your work All in 3 minutes or less—read today's Wildtype Weekly 👇

“Everyone does it this way” is not a valid statistical defense. (a thread)

AI still struggles to replace researchers because of one trait that machines still lack Researchers who use it consistently reach world-class levels 🏆 What is this trait? Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)

While irreproducibility is undermining the biggest research breakthroughs We're hearing more false data today than ever The literature is being taken with a pinch of salt This one phrase in the methods section gave a confidence boost for the whole article (1/4)

Bench problems AI already solved—and what AI still cannot do 🥼 (a thread)

Good news: you don’t always have to repeat the experiment — because your next figure might be hiding in your old data (a thread)

Researchers, don't wing your statistics 📊—here's the best software for your lab 🔬 (a thread)

Imagine your experiment worked—but it’s invalid because you didn't the correct control! How do you avoid choosing the wrong control? Ready today's Wildtype Weekly to learn more (in 3 minutes or less 👇)

Part 2/2—let’s finish the list! 3 pro-tips from a decade of flow experience (in 3 minutes or less) Read today's Wildtype Weekly 👇

All of the following do NOT count as sufficient controls (by lab technique) (a thread)

A tip from over a decade of flow cytometry experience: (a thread)

We all had to repeat failed experiments because our first design wasn't optimal. How to avoid this in flow cytometry? Read Juliano's six panel design pro-tips in 3 minutes or less in today's Wildtype Weekly 👇

Researchers, Flow data feels objective, until two people gate it (1/4)

Humble, precise language can make your lab work shine But when describing their lab work, researchers often misuse the same 10 words Are you using them too? Read the Wildtype Weekly to find out (in 3 minutes or less 👇 )

FlowJo should not be the default! 🙅‍♂️ How can you choose the best flow cytometry software for your case? Here's your rule of thumb: (1/9)

Researchers, don't choose the default just because everyone uses it. Here's how to pick the ideal flow software for your case (see today's post on LinkedIn 👇) www.linkedin.com/posts/wildty...

Researchers, If your blot only works in your hands, it’s not evidence yet. The silent killer? Antibody lot change (1/3)

Remember: Peer review is a human process, and some comments can harm your work 🔬 Wildtype One looked at true stories from researchers We found 10 reviewer comments you should absolutely ignore Read today's Wildtype Weekly to find them out 👇

Quick tip 🔬🧑‍🔬 If you do Western blots, immunofluorescence, or any technique that requires antibody staining Remember: Overnight incubation at 4°C is not always the best protocol ⚠️ (1/2)

Do you use SEM because it looks cleaner? SEM vs. SD = one of the most quietly damaging figure-making habits in biology Let’s clear it up (a thread)

Many cell lines in research aren't what researchers think they are 🧫 So, how would a world-class scientist troubleshoot their project? Read today's Wildtype Weekly to find out (in 3 minutes or less 👇)

Researchers, Most irreproducible results don’t fail loudly They quietly rot (a thread)

Sadly, only 11-25% of the biology literature is reproducible But there's a solution... (a thread)

Reviewers will ask you to repeat experiments... Only because of small details that you didn't include How do you avoid losing time and repeating experiments? 🤔 (a thread)

Could your cell line be the wrong one for your disease? 🧫 (a thread)

“There are 5 rules. People tried to bend them, and the data punished them for it.” 🫣 Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice. What are his rules? Discover them in today's Wildtype Weekly (in 3 minutes or less 👇)

Everyone’s thinking it. Few say it out loud. The literature looks confident - Clean figures - Tight conclusions - Cropped blots that “say enough” (1/n)

How to choose the ideal figure type to your experiment 🥼🔬: (a thread)

How can you publish in high-impact journals? Here's advice from a Nature Editor (a thread)