Sadly, only 11-25% of the biology literature is reproducible But there's a solution... (a thread)

Reviewers will ask you to repeat experiments... Only because of small details that you didn't include How do you avoid losing time and repeating experiments? 🤔 (a thread)

Could your cell line be the wrong one for your disease? 🧫 (a thread)

“There are 5 rules. People tried to bend them, and the data punished them for it.” 🫣 Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice. What are his rules? Discover them in today's Wildtype Weekly (in 3 minutes or less 👇)

Everyone’s thinking it. Few say it out loud. The literature looks confident - Clean figures - Tight conclusions - Cropped blots that “say enough” (1/n)

How to choose the ideal figure type to your experiment 🥼🔬: (a thread)

How can you publish in high-impact journals? Here's advice from a Nature Editor (a thread)

So, should you switch to spectral cytometry? Not before you know a few basic principles. Juliano Freitas, Ph.D., has over a decade of flow cytometry experience—and some good advice. Read what he'd advise you in today's Wildtype Weekly (in 3 minutes or less 👇)

Researchers, how would you handle your "non-significant" statistics? 🧫📊 Vote here 👉 www.linkedin.com/feed/update/...

The top 5 Western blots failures researchers complained about online in 2025 🧻 (a thread)

Your Western blot shouldn’t take two days (a thread)

We're researchers. We're paid to think and discover—not solve technical issues. To make 2026 more productive, we made a list of affordable lab tools that solve the most annoying problems (for less than 1,000€) Read the last Wildtype One weekly of 2025 🍾 👇

What if a statistician ran a cancer cell-biology lab? 🧫 🧬 (...and what can we learn from them?) (a thread)

Researchers, T-tests are often misused—here’s a quick rule of thumb 👇 (a thread)

Hot take: 96-well plates are NOT inherently high throughput 🧫 (a thread)

If you're a young researcher, there is still a lot to learn about flow cytometry 🤕 Today, we decided save you some of the trial and error We compiled 7 veteran flow cytometry tips (that beginners learn the hard way) Read today's Wildtype Weekly (in 3 minutes or less)👇

This is so important, it's worth repeating: Isotype control isn't your best negative control in flow cytometry (a thread)

Not all articles reproduce. How do you make sure yours does? Read today's Wildtype Weekly (3 minutes or less 👇)

"The reason I quit science was because of an antibody not working!" On the ProteinTech Podcast, Dr. Anita Bandrowski shares a story She had a colleague who was working with an antibody manufacturer Here's the story she shared (a thread)

Researchers, what would you do with your magic wands? 🪄 Vote here 👉 www.linkedin.com/feed/update/...

This is almost unbelievable But cell density is a silent trap (a thread)

A quick tip from Wildtype One: Stop using pie charts Especially when you're showing evolution, like: - "Before vs. After" - "2020 vs. 2025" - Flow cytometry cell population changes ❌ Pie charts are hard to read (1/2)

Researchers: It’s the end of the year—this means expiring licences Do you have a plan to avoid work interruption? Save cost? Choose the best software? We found a solution Read today's Wildtype Weekly in 3 minutes or less 👇

What gift should you buy your labmates for Christmas? 🎅🔬 Here are 9 budget-friendly Secret Santa ideas for researchers 🎁👇

You’ve probably heard nutritionists say: "If the first slice of bread is moldy, you shouldn’t just throw it away and eat the rest" "The whole bag may already be contaminated" Same thing with cell culture (but not always!) ... (1/n)

No—not all filaments in cell culture are fungi (1/n)

Yes, you can use AI for academic writing—as long as you follow the rules Here is how to ethically use AI in academic writing: (key takeaways—a thread 👇)

4 Western blot "non-specific" bands you're cropping out... that are actually valuable signal! (a thread)

Researchers, Retractions can come from innocent (& avoidable) mistakes Part 1 of this series was real-life examples Part 2 was honest mistakes that count as misconduct Part 3 (today): Your simple checklist to avoid misconduct issues Read today's Elite Researcher's Weekly, in 3 minutes or less 👇

4 microscopy “artifacts” you're throwing away ...that are actually valuable signal! (a thread)

How can you choose the best fluorescent dye combinations in seconds? We found a useful online tool that will help you: (a thread)

“Where’s the damn question?!” If Richard Feynman... (yes, the great physicist) ...were your PI (yes, in a biology lab) He'd definitely go over your notebook: (see thread)

Flow cytometry 🔬 and urinals 🚽 have something in common... Below is a veteran flow cytometry concept …that beginners don't know (a thread)

Researchers 🥼, Retractions can come from innocent mistakes Luckily, they're avoidable We created a series Part 1 was real-life examples Today is Part 2: Honest mistakes that count as misconduct Read today's Elite Researcher's Weekly, in 3 minutes or less 👇 (...and stay tuned for Part 3 ⌛)

AI taking jobs ❌ AI changing lab standards ✅ Not all lab techniques will make it to 2026 Here are the techniques that will lose their "standard" status by 2026 (see reply 👇)

Researchers, What to do when your licence expires? How do you: ... avoid work interruption? ... save cost? ... choose the best software? (a thread)

Reminder to researchers doing Western blot: Bacteria and mold can contaminate your TBST too And this ruins your Western blots The worst part? (thread)

The most iconic moments in the history of biology (a thread)

Researchers, Misconduct can be unintentional It could be a mistake on the bench (or in the office) I.e., it's avoidable We created a series around this topic This is Part 1 Read this week's Elite Researcher's Weekly, in 3 minutes or less 👇 (...and stay tuned for Part 2 ⌛)

Choosing the wrong test can cost you statistical power. Here's how to decide: (a thread)

Once again, I beg you to understand that SINGLE-CHANNEL FLUORESCENCE MICROSCOPY IMAGES DON'T HAVE A COLOR!

Not all lab techniques will make it to 2026 Labs evolve fast Habits don’t Many “standard” methods are no longer necessary Better, more powerful options are replacing them Here are some examples 👇 (a thread)

"What should I do after the PhD?" "Can I find a job?" "Will I get the position I want?" … Here's some good news:

One of the most legendary papers in molecular biology was written by a postdoc... He didn’t even mean to create it. 🧬 Quick backstory—1970 Ulrich Laemmli was a young postdoc at the MRC-LMB in Cambridge. (a thread)

Many young researchers believe the myth that isotype control is the best negative control. Here's the truth 👇

Do you agree?

What do you think matters most when sending your draft to Nature? Surprise: It's not your track record! Heathcliff Dorado García (PhD and Nature Portfolio Editor) shared a few nuggets on publishing in prestigious journals ✍ Read today's Elite Researcher Weekly for more 👇

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass... I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands) #WesternBlot #cellsky

Here's a very bad flow cytometry advice: “autofluorescence needs to be minimized” Analogy: Imagine you're watching TV and you have static... (a thread)

Albert Einstein would've hated Western blots... (a thread)