John Blair
@jblairsci.bsky.social
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📥 15
📝 30
Postdoc in the Satija Lab at NYGC. Former PhD at Bateup Lab at UC Berkeley
Check out our new paper that
@abradu.bsky.social
does a great job of explaining here!!! If you have any questions or want to try to use this tech please reach out!!!
add a skeleton here at some point
3 months ago
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The second paper I want to highlight was led my Izzy Grabski, where she developed a new method of "RNA Fingerprinting" to map transcriptional responses to perturbation dictionaries
www.biorxiv.org/content/10.1...
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Mapping transcriptional responses to cellular perturbation dictionaries with RNA fingerprinting
Single-cell perturbation dictionaries provide systematic measurements of how cells respond to genetic and chemical perturbations, and create the opportunity to assign causal interpretations to observa...
https://www.biorxiv.org/content/10.1101/2025.09.19.676866v1
7 months ago
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I wanted to highlight two recent papers from myself and colleagues! In the first, we develop a method, FlexPlex, that leverages the 10x Genomics Flex kits to capture RNA, intracellular protein and guide RNAs in single cells with high resolution multiplexing
www.biorxiv.org/content/10.1...
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Iterative, multimodal, and scalable single-cell profiling for discovery and characterization of signaling regulators
Cell signaling plays a critical role in regulating cellular state, yet uncovering regulators of signaling pathways and understanding their molecular consequences remains challenging. Here, we present ...
https://www.biorxiv.org/content/10.1101/2025.08.27.672635v2
7 months ago
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Excited (again) to see this paper finally in (pre)print! We did single-cell sequencing on our TSC model organoids and observed strong signatures of reactive astrogliosis (neat)- then we looked at resected patient brain tissue and saw the same thing! (More neat!)
www.biorxiv.org/content/10.1...
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mTORC1 activation drives astrocyte reactivity in cortical tubers and brain organoid models of TSC
Tuberous Sclerosis Complex (TSC) is a genetic neurodevelopmental disorder associated with early onset epilepsy, intellectual disability and neuropsychiatric disorders. A hallmark of the disorder is co...
https://www.biorxiv.org/content/10.1101/2025.02.28.640914v1
about 1 year ago
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Happy that my first paper from my Postdoc is out where we perform Phospho-seq, a single-cell ATAC-sequencing with intracellular protein quantification in human brain organoids:
www.nature.com/articles/s41...
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Phospho-seq: integrated, multi-modal profiling of intracellular protein dynamics in single cells - Nature Communications
Here, the authors demonstrate Phospho-seq, a single-cell multiomics method capable of quantifying chromatin accessibility alongside intracellular proteins, including post-translationally modified prot...
https://www.nature.com/articles/s41467-025-56590-7
about 1 year ago
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