#WiMiBiotech is showcasing its high-efficiency plant genetic transformation and precision crop gene-editing platforms as a proud sponsor at ICAR 2026 in Singapore—visit us at Booth 7 (Seminar Room 1/2) to supercharge your research! 🇸🇬🌱#ICAR2026 #Arabidopsis #PlantScience #GeneEditing #FutureBreeding

Stanford and Arc Institute developed #Proto, a groundbreaking high-level programming language for generative biology that unifies diverse #AI models into four core primitives, allowing researchers to compile completely synthetic, functional DNA-protein regulatory networks using natural language. 🧬🤖

#PREMIER, a groundbreaking DSB-free technology that uses a quad-pegRNA array to generate single-stranded microhomology flaps, driving precise #genomicreplacements of up to 10.3 kb fragments with a peak efficiency of 85.9% in mammalian cells and live animal models.

UC researchers bypassed the traditional low-MOI screening dogma by using medium-high MOI 5 to co-deliver multiple sgRNAs per cell, allowing robust genome-wide #CRISPRi screens to be successfully executed using only 500,000 sorted cells. 📊🤖 #HighThroughput #Genomics #ScreenCompression #NatureMethods

Split crRNA:tracrRNA architectures can be hijacked by host RNAs to cause a hidden form of #CRISPR off-targeting, and solved this by training machine-learning models to design highly secure, non-repetitive scaffolds that prevent self-editing and enable safe 6-gene multiplexing. 🧬🤖

Researchers engineered an ultrasound-actuated peptide nanoemulsion (NPep) system that triggers micro-bubble phase transitions in tissue interiors, ballistically injecting CRISPR RNPs 12–20 μm deep into human kidney organoids with exceptional safety. 🔊🤖 #GeneEditing #Ultrasound #Organoids #BioTech

David R. Liu's team achieved a breakthrough 49% in vivo #primeediting efficiency in mouse liver by systematically optimizing #LNP cargo stoichiometry, HPLC-purifying the #mRNA, and utilizing exonuclease-resistant 3' epegRNA motifs to successfully reverse the Phenylketonuria disease phenotype. 🧬🤖

Perturb-DBiT, an ingenious microfluidic spatial screening platform that simultaneously maps genome-wide #CRISPR perturbations and total non-coding transcriptomes on the same tissue section, preserving spatial clone architectures and immune niches at near-single-cell resolution. 🖼️🤖 #SpatialOmics

First endogenous U6 promoters cloned for #kenaf: native HcU6-14P drove 28.5% #CRISPR editing at the ALS locus with 100% positive transformation, while a foreign cotton GbU6-9P promoter produced zero mutations despite 71.4% transformation success — a clear case for species-specific sgRNA promoters.

Scientists engineered a high-precision, near-PAMless cytosine #baseeditor by combining a truncated CDA1 deaminase with an HMGN1-VP64 dual-fusion architecture, tightly locking the conversion window onto the C18 position and achieving a peak editing efficiency of 78.57% in stable #rice lines.

Chinese synthetic biologists engineered the PE7-scFv-MLH1dn system using SunTag spatial scaffolding to anchor mismatch repair inhibitors directly to active DNA nicks, lifting prime editing efficiencies by 68% without introducing background genomic damage. 🧬🤖#SynBio #CRISPR #PrimeEditing #SunTag

CAAS scientists broke through the plant #primeediting size ceiling by developing the quadruple-mutant #rPE14e4 reverse transcriptase and template-jumping mechanics, achieving a 4.5-fold efficiency boost and enabling precise 250 bp isogenic sequence replacements in elite rice lines. 🌾🤖 #Rice

🌾 iSpyMacCas9-Combo activates OsBBM1 12× in rice, enabling 42% hormone-free regeneration, while simultaneously boosting multiplex editing — including previously uneditable AT-rich loci — with 15/19 T0 plants achieving dual or triple knockouts; #Cas12b-Combo failed at only ~3× activation.

🌿 Head-to-head CRISPRa in N. benthamiana: at the same pNOS target, dSaCas9:EDLL+MCP:VPR achieves ~18× induction vs. ~2× for dSpCas9 — and adding more actRNA copies doesn't help, with guide position being the dominant factor. BMC Plant Biology | Rischer lab, VTT. #CRISPRa #PlantBiology #Cas9

Chinese scientists successfully developed the #gMDE toolbox by exploiting plant-specific AP lyase DNA repair pathways, converting base editors into precise multi-nucleotide deletion processors to engineer 6–20 bp crop non-coding regulatory elements for massive yield increases. 🌾🤖 #PromoterEditingç

#ColumbiaUniversity researchers achieved the first precise base editing in human embryos using #ABE #RNPs with zero chromosomal damage, though a 78% mosaicism rate and commercial ties to embryo "optimization" have ignited an immediate global bioethics storm. 👶🤖 #BaseEditing #CRISPRHistory

#UCLA researchers successfully bypassed the tissue-culture bottleneck in tomato by using #Tobacco Rattle Virus to deliver the ultra-compact #TnpB nuclease directly into wound-induced newborn shoots, producing 100% transgene-free progeny with a 30% increase in fruit weight. 🍅🤖 #tomato

The new twin-prime-editing-based #TRIM platform achieves a 4.2-fold efficiency leap over #Cas9 by substituting error-prone double-strand breaks with predictable stop-codon insertions, enabling simultaneous multi-gene knockouts, single-base swaps, and 4.9-kb large #DNA insertions in #monocots. 🌾🤖

David R. Liu's team developed the #PE8 series by using AlphaFold and #ProteinMPNN to structurally redesign reverse transcriptases, boosting protein stability by up to 8°C and delivering up to a 3.5-fold efficiency leap in vivo without sacrificing editing specificity. 🧬🤖

2026 Roadmap: Disruption of the #tomato histone demethylase SlLDL1 lifts heterochromatin silencing on a truncated B-type cyclin cluster, driving ectopic cell proliferation that simultaneously triggers excessive adventitious rooting and rough fruit skin. 🧬🛡️ #PlantScience #CRISPR #Epigenetics

🌿 #CRISPR knockout of MeSSI in #cassava shifts starch chain distribution, boosting resistant starch 6.74× (1.46% → 9.85%) and amylose +16.42% — with total starch, tuber size, and yield completely unchanged, thanks to transcriptome-confirmed compensatory upregulation of other starch synthases.

🫛 CRISPR knockout of GmSH2a + GmSH2b in vegetable soybean blocks AGPase, drops starch ~40%, and redirects carbon to soluble sugars and protein — sweeter, more nutritious edamame with zero change in yield, pod number, or grain size. Plant Physiology | Li et al. #Edamame #Soybean #CRISPR

2026 Roadmap: A definitive study demonstrates that tapetum-specific NEF1 acts as a critical ER-Golgi transporter for AGPs and polysaccharides, where its disruption causes total primexine template collapse and 100% male sterility. 🧬🛡️ #PlantScience #CRISPR #MaleSterility #HybridBreeding

2026 Roadmap: A newly established #broccoli protoplast regeneration platform utilizes Red + Far-Red #LED lighting to trigger phytochrome signaling, boosting shoot regeneration efficiency eightfold compared to blue light within a 5-month workflow. 🧬🛡️ #CRISPRChassis #ProtoplastRegeneration

🧬 Fusing ATPase DnaA to PlmCas12e via a rigid N-terminal linker lowers its conformational activation barrier — boosting editing 1.34× across endogenous loci. Preprint | Liu lab, Tsinghua. #CRISPR #Cas12e #ProteinEngineering

🧬 #CRISPR-MACE uses adenovirus replication + error-prone polymerase + tunable anti-CRISPR pressure to continuously evolve #Cas9 directly in human cells — yielding DVKIH, a 5-mutant variant with 3× higher DNA affinity and ~1,000× anti-CRISPR resistance, validated in #CASFISH imaging.

🌾 Cas12i.3 dual knockout of PmBADH2a + PmBADH2b creates the first-ever fragrant broomcorn millet — 6.83 mg/kg 2-AP in transgene-free double mutants, with no change in yield, heading date, or grain size. JIPB | Song lab, CAU. #Millet #CRISPR #CropQuality #Fragrance

🌲 AtMYB2 directly represses AtMYB48 and AtbHLH68 to suppress cambium proliferation while activating wood differentiation — but the two downstream factors are functionally redundant yet specialized (AtMYB48: secondary wall/PCD; AtbHLH68: cambium proliferation) #WoodFormation #Cambium #PlantBiology

🌾 CRISPR dual knockout of SbMATL + SbDMP creates the first commercially viable haploid inducer line in sorghum (up to 16.04% induction), with GFP+RUBY visual screening and spontaneous chromosome doubling — compressing 6–8 generations of selfing into one season. #Sorghum #DoubledHaploid #CRISPR

2026 Roadmap: The #PrEditR platform streamlines functional proteomics by allowing researchers to design #baseeditor sgRNAs directly from protein residue coordinates through an automated, isoform-aware mapping architecture. 🧬🛡️

🍅 Five #CRISPR edits across four metabolic pathways simultaneously boost #Vitamin D3 (0→0.70 μg/g), Vitamin C (2.53×), lycopene (7.07×), β-carotene (3.86×), and GABA (5.26×) in transgene-free #tomatoes — with confirmed anti-colorectal cancer activity in vitro and in vivo, zero yield penalty.

🧬 TAMIPAMI maps PAM/TAM sequences for Cas9, Cas12, and TnpB with just one library pair (no time-series), 100% match to published motifs, z-score cleavage quantification, and automatic IUPAC output — all free in a browser at tamipami.che.ufl.edu. bioRxiv. #CRISPR #PAM #TnpB #Bioinformatics

🌿 TadA8e-derived GhTad-CDd achieves ~80% average C-to-T editing in cotton, while GhTadDE simultaneously delivers C-to-T and A-to-G edits at up to 85.95% total efficiency — with GhWOX1-edited T0 plants showing diverse leaf shapes confirmed by transcriptome rewiring. #BaseEditing #Cotton #CRISPR

2026 Roadmap: Researchers established the first stable #CRISPR/Cas9 system in Physalis grisea by targeting the #PgPDS gene, enabling highly efficient, visual screening of mutant albino plantlets without primary PCR diagnostics. 🧬🛡️

🫘 Optimized vacuum-assisted Agrobacterium infection + GFP screening achieves 6.5% stable transformation in common bean, while a paired hairy root system delivers ~100% #CRISPR editing efficiency for rapid target validation.Plant Cell Tiss Organ Cult | Ke et al. #CommonBean #PlantBiotechnology

🧬 CleanFinder is a browser-native, no-code framework that classifies #PE/BE editing products at single-nucleotide resolution, infers NHEJ vs MMEJ repair, detects allele dropout via SNP barcoding, scales to 1,849-compound screens, and runs entirely locally — no data upload needed.

🌾 TREX2-SpCas9 + a 29-module tRNA-gRNA array simultaneously knocked out all 29 OsCPK family genes in individual #rice plants, with 83% of T0 plants carrying 19–27 simultaneous edits — enabled by screening 38 rice tRNAs and identifying 13 high-efficiency elements (two hit 100%). #MultiplexEditing

🌿 Directly fusing Csy4 to the prime editor (uncleavable) instead of using a P2A linker reduces toxicity 13.5× in Arabidopsis and unlocks up to 85.7% T1 editing efficiency — decoupling Csy4's efficiency boost from its lethality in dicots. #PrimeEditing #Arabidopsis #CRISPR

🌾 ABE8e converts SbALS W545→R545 in sorghum (11.6% in protoplasts, 33% in T0 plants), creating heterozygous lines that survive 8× the lethal imazethapyr dose in field trials with no agronomic penalty — the second back-to-back sorghum base editing paper from CAAS. #BaseEditing #Sorghum #CRISPR

🌾 First prime editing system in sorghum: fusing T5 exonuclease to nCas9+M-MLV (SbPEmaxT5E) boosted ALS W545L editing to 9.33% in protoplasts and 6.25% in stable plants — with transgene-free T1 lines confirmed herbicide-resistant in greenhouse trials. #PrimeEditing #Sorghum #CRISPR

🌿 CRISPR dual-copy knockout of BnaLYK — the rapeseed orthologue of AtLYK1 — abolishes chitin/PGN-triggered ROS burst, callose deposition, and MAPK signaling, confirming it as an essential PTI receptor and validated breeding target for Sclerotinia resistance. #Rapeseed #PlantImmunity #CRISPR

🌾 Knocking out a single α-prolamin gene in foxtail millet boosted lysine by 89%, GABA up to 4×, and all 7 essential amino acids to WHO/FAO standards — with improved flavor and zero yield penalty. Plant Cell Reports | Zhao et al. #CRISPR #FoxtailMillet #CropNutrition

🧠 Liu lab's ABE corrects the Dravet syndrome SCN1A R613X mutation in vivo — 97% transcript correction, restored interneuron firing, zero spontaneous seizures, survival up from 27% to 90%. Science Translational Medicine. #BaseEditing #GeneTherapy #Dravet

2026 Roadmap: By editing #CENH3 within a cytoplasmic male sterile background, researchers established a one-step CMS introgression system in #rapeseed, eliminating 6–8 generations of traditional backcrossing through paternal haploid induction. 🧬🛡️

🧬 QuadPE (Nature) uses four pegRNAs to insert up to 26 kb with ~40% efficiency — no DSBs, no recombinase — outperforming integrase systems by up to 61× and working in primary neurons and T cells. Yin/Zhang labs, Wuhan University. #PrimeEditing #GeneTherapy #CRISPR

🌱 #GmSNAP14 is confirmed as a soybean SCN susceptibility gene: #CRISPR #knockout boosts resistance to virulent HG type 1.2.5.7, two natural loss-of-function alleles explain 62.4% of resistance variation, and TaqMan markers are ready for breeding.

✈️ The sustainable aviation fuel #crop #Brassicacarinata finally has a reproducible, #genotype-independent transformation system — 5–12% stable efficiency across 4 genotypes, with the surprise finding that regeneration ability doesn't predict transformation efficiency.

Excited to announce that WiMi Biotechnology is a proud sponsor of the 4th International Wheat Congress (#IWC2026) in Bologna, Italy! 🇮🇹🌾 See you there! 🤝 #WheatInitiative#PlantBiotech #CropScience #GeneEditing #CRISPR #Agriculture #WheatResearch #Bologna

🧬 Doudna lab found a pre-CRISPR RNA-guided DNA system — 20 kD, no PAM, targets wobble positions via a 1nt-skip rule that makes viral escape nearly impossible. bioRxiv. #CRISPR #VIPR #GeneEditing

🤖 One round of DNA shuffling + ML virtual screening of 10M sequences → base editors that beat ABE8e on activity, off-target, and size — no extra rounds of directed evolution needed. bioRxiv | Bowen lab, UCB. #BaseEditing #MachineLearning #CRISPR