As 2025 draws to a close, we at Pensoft want to extend our warmest wishes to our editors, reviewers, researchers, partners, and followers. Thank you for being part of our journey this year. Wishing you all a joyful holiday season and a bright start to the new year! 🎄✨

Day three of #BES2025 is underway! Find us at booth L12 in the Lennox Suite.

This baseline study explores meiofauna diversity in Baltic Sea marine protected areas and adjacent reference areas using multi-gene metabarcoding and morphology - key for tracking trawling ban impacts​. 🔗 doi.org/10.3897/mbmg... @sgn.one

Across 21 European lakes, metagenomes and metatranscriptomes reveal different, method-dependent pictures of microbial diversity and environment links, proving that sequencing choice can alter ecological conclusions. 🔗 doi.org/10.3897/mbmg... @unidue.bsky.social

Integrating legume-based green manure and organic amendments in barley cropping systems enhances rhizosphere microbial diversity and resilience, promoting sustainable soil health and productivity. 🔗 doi.org/10.3897/mbmg...

Come to the Pensoft stand at the Kliments' Days 2025 in Sofia, Bulgaria! Our Marketing and Sales manager @maria-kolesnikova.bsky.social is there to welcome you and tell you all about our open-access journals in biodiversity sciences!

A targeted DNA metabarcoding workflow was developed and validated to identify terrestrial gastropods from the digestive contents of the invasive flatworm 𝑂𝑏𝑎𝑚𝑎 𝑛𝑢𝑛𝑔𝑎𝑟𝑎, revealing diverse prey and improving diet analysis. 🔗 doi.org/10.3897/mbmg...

eDNA metabarcoding outperforms traditional electrofishing for monitoring fish and decapod biodiversity in Caribbean freshwater ecosystems, offering a less disruptive, more comprehensive method to detect native and invasive species and assess ecological health. 🔗 doi.org/10.3897/mbmg...

Stemflow for detecting mammalian environmental DNA: a case study in a zoo. 🔗 doi.org/10.3897/arph... @mbmg.pensoft.net

DNA barcoding reference dataset revealed overlooked diversity and clarified biogeography of fishes in the largest European river system – Volga River. 🔗 doi.org/10.3897/arph... @mbmg.pensoft.net

Our journal is at #LivingData2025 - looking forward to talking to anyone interested in #metabarcoding and metagenomics!

Taxon-specific mini-barcode primers greatly improve the detection and biodiversity assessment of key marine zooplankton groups, boosting species representation by up to fivefold. 🔗 doi.org/10.3897/mbmg...

Guidance and best practices for species identification using eDNA metabarcoding - When do you call a cod a cod? 🔗 doi.org/10.3897/arph... @mbmg.pensoft.net

Multiplex PCR sequencing represents a powerful and cost-effective tool for simultaneously detecting multiple aquatic invasive species in the early stages of invasion: doi.org/10.3897/mbmg...

"Want to start using Nanopore technology to research protistan diversity? Check out our paper introducing a pipeline for creating OTUs from Nanopore metabarcodes — bridging the gap between short- and long-read metabarcoding." - Anna Karnkowska doi.org/10.3897/mbmg...

Non-invasive swabbing of hive entrances is a highly effective biomonitoring method for collecting hive eNA and delivering comprehensive surveillance for animal, plant and microbial communities in the environment. 🔗 doi.org/10.3897/mbmg...

A 16S rRNA dataset of Las Tablas de Daimiel National Park. 🔗 doi.org/10.3897/arph... @mbmg.pensoft.net

Optimised eDNA and PCR-based metabarcoding significantly outperformed hybridisation capture and traditional field surveys for bryophyte detection in river ecosystems. 🔗 doi.org/10.3897/mbmg...

Researchers present the BaNaNA (Barcoding Nanopore Neat Annotator) pipeline and display the effectiveness of long-read Nanopore sequencing for protist biodiversity, ecology and evolution research. 🔗 doi.org/10.3897/mbmg... @ankarn.bsky.social @fabnot.bsky.social

DNA metabarcoding identifies more invertebrate species under the Water Framework Directive than conventional methods, showing promise for faster and finer river health monitoring in Slovakia’s Carpathian streams. 🔗 doi.org/10.3897/mbmg...

Tiina Laamanen @tiinalaamanen.bsky.social cited her MBMG paper on molecular biodiversity monitoring methods as part of her #BGEconnects session on implementing #eDNA and other molecular monitoring methods in Finland!

After more than fourteen years of advancing research on biological invasions, we have published our 100th issue! 🌎 🔗 blog.pensoft.net/2025/09/29/a...

DNA barcoding reveals that the diversity of 𝑁𝑖𝑝ℎ𝑎𝑟𝑔𝑢𝑠 in France is dramatically underestimated. 🔗 doi.org/10.3897/zse....

📰 A recent article, supported by our project, was published in Pensoft Publishers' (@pensoft.net) Metabarcoding and Metagenomics journal. 🧬 The study evaluates the TRLs of molecular tools for #monitoring like #eDNA and DNA #metabarcoding. 👀 Full article: mbmg.pensoft.net/article/1308...

The latest guest post on the @pensoft.net blog outlines a new, non-lethal method of studying pollinator networks. 🐝🧬 blog.pensoft.net/2025/09/11/t...

The latest guest post on the @pensoft.net blog outlines a new, non-lethal method of studying pollinator networks. 🐝🧬 blog.pensoft.net/2025/09/11/t...

Using molecular and morphological evidence, researchers present the first occurrences of the non-indigenous diatom species, 𝐷𝑖𝑠𝑐𝑜𝑠𝑡𝑒𝑙𝑙𝑎 𝑎𝑠𝑡𝑒𝑟𝑜𝑐𝑜𝑠𝑡𝑎𝑡𝑎 in the Danube River and the earliest occurrence in European freshwater ecosystems. 🔗 doi.org/10.3897/mbmg...

🌎 Potential of simple, replicable methods to support long-term #monitoring demonstrated at a workshop of the #Marine #Biodiversity Observation Network Pole to Pole of the Americas that brought together participants from 10 countries earlier this year. 📑 Workshop Report: doi.org/10.3897/rio....

By comparing three strategies for local bioassessments in streams using environmental DNA and RNA metabarcoding, researchers find the most effective strategy is to briefly incubate collected organisms in water then analyse the enriched eDNA. 🔗 doi.org/10.3897/mbmg...

Researchers utilise DNA metabarcoding to reveal distinct trophic niches among insectivorous bird species in central Peninsular Malaysia, highlighting the need to conserve insect-rich habitats within mixed-use landscapes. 🔗 doi.org/10.3897/natu...

Combining culture-based approaches with molecular phylogenetics and eDNA metabarcoding, researchers uncovered two new fungal genera within the Lulworthiales, expanding the known ecological range of this order from marine to terrestrial saline habitats. 🔗 doi.org/10.3897/imaf...

Researchers demonstrate the effectiveness of DNA metabarcoding in assessing aquatic invertebrate diversity across an altitudinal gradient in the Tatra Mountains. 🔗 doi.org/10.3897/mbmg...

COI barcoding is effective for species identification in 𝑃𝑎𝑐ℎ𝑦𝑔𝑟𝑎𝑝𝑠𝑢𝑠 crabs and in unravelling intraspecific patterns indicative of cryptic diversity guiding further taxonomic revisions integrating morphological, genetic and ecological data. 🔗 doi.org/10.3897/mbmg...

Using light microscopy and metabarcoding, researchers have revealed microalgae diversity in yellowfever mosquito (Aedes aegypti) larvae guts and breeding sites in Thailand, providing potentially valuble information for biocontrol. 🔗 doi.org/10.3897/mbmg...

"Many people think shotgun metagenomes only pick up bacteria, but in fact we detected many vertebrates in marine samples including birds, fish, and mammals! This PCR-free approach may one day be the key to better biodiversity surveys." 🔗 doi.org/10.3897/mbmg...

Excited to share my new study comparing shotgun metagenomes with metabarcoding for detecting eukaryotes in the ocean! Out today in @mbmg.pensoft.net mbmg.pensoft.net/article/1449...

📑Latest addition to the #openscience collection of the #European Reference #Genome Atlas (ERGA, @ergabiodiv.bsky.social) presents a the 1st #genome report for a sun spider (order Solifugae). 🔗 Find the report: doi.org/10.3897/arph... 🔍 Explore the collection to date: doi.org/10.3897/rio....

📸 This week, our consortium at the Bulgarian Barcode of Life, #BgBOL, together with @biogeneurope.bsky.social & @iboleurope.bsky.social, we organised the 1st practical course on #DNAbarcoding & #sequencing, targeted at early-career scientists. ℹ️ Read more about the event: bgbol.org/en/news/prac...

Metabarcoding and Metagenomics has received its first Journal Impact Factor! mbmg.pensoft.net/news/917

Very good to see our @pensoft.net journal @mbmg.pensoft.net continues to receive good CiteScores and also now the first #ImpactFactor with a good value of 3.1 ☀️ While I personally don’t care much about these metrics I know they are really relevant for many institutions. Thus: very happy about this!

DNA metabarcoding shows that the notch-eared bat’s diet, dominated by spiders and livestock pest flies, is shaped by local land cover and farming practices across southwestern Europe. doi.org/10.3897/mbmg...

A new Scopus CiteScore for Metabarcoding and Metagenomics. mbmg.pensoft.net/news/884

Vist the @pensoft.net stand (#27) at the International Congress for Conservation Biology in Brisbane/Meanjin, Australia! 🔗 mbmg.pensoft.net/news/863 📅 15-19 June 📍 Brisbane Convention & Exhibition Centre @iccb2025.bsky.social

Microbial communities in the sediments of six remote coastal dune lakes on Chatham Island showed distinct taxonomic and functional differences. 🔗 doi.org/10.3897/mbmg...

Researchers demonstrate a non-lethal method for DNA sampling from bumble bees, enabling plant-pollinator network analysis via barcoding and metabarcoding without harming insect populations. 🔗 doi.org/10.3897/mbmg...

Follow our portfolio of OA, peer-reviewed journals to keep up to date with the latest research in biodiversity, ecology, and more! go.bsky.app/VKsmRtL

Happy #WorldBeeDay! In a recent study in our journal, researchers used pollen #metabarcoding to analyse bee specimens, revealing significant shifts in foraging patterns. Learn more on the @pensoft.net blog: blog.pensoft.net/2023/07/11/n...

🧬 Calling all #eDNA and #EnvironmentalOmics enthusiasts! At the AquaEcOmics meeting, 35 international experts discussed forming a European Society for Environmental Omics (ESEO) to strengthen collaboration across the field. 🧵(1/4)

👋 Greetings from the @egu.eu General Assembly in Vienna! 🇦🇹 👌 Great occasion to spread the word about the #Biodiversity Knowledge Hub: a major outcome of the #BiCIKL project dedicated to #FAIRdata in biodiversity we coordinated! 👉 Read more on our blog: blog.pensoft.net/2025/04/30/b.... #EGU2025

The citizen science event BioBlitz 2025 united experts and enthusiasts in a special initiative to study the biodiversity of Borisova Garden in Sofia, Bulgaria. mbmg.pensoft.net/news/844