Johan Slotman
@johanslotman.bsky.social
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Super-Resolution Microscopy | Erasmus MC Optical Imaging Centre Rotterdam | www.erasmusoic.nl
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So lets try bluesky as well for science. I'm an assistant professor focusing on advanced fluorescent imaging and image analysis and part of the Erasmus MC Optical Imaging Centre core facility.
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10 months ago
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reposted by
Johan Slotman
Selçuk Yavuz
22 days ago
Wondering how a fully automated confocal microscopy setup could help biologists with rapid acquisition of quantitative imaging data? Check now our new article published in
#FEBS
Open Bio:
febs.onlinelibrary.wiley.com/doi/10.1002/...
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FEBS Press
RoboMic is an automated confocal microscopy pipeline for high-throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI-driv...
https://febs.onlinelibrary.wiley.com/doi/10.1002/2211-5463.70161
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Getting SMART about FRAP enabled us to make many FRAP curves in one imaging session. Now we are doing things like 18 conditions n = 30 in a single overnight session. Study protein dynamics in high throughput. Curious on how? see our publication:
febs.onlinelibrary.wiley.com/doi/full/10....
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FEBS Press
RoboMic is an automated confocal microscopy pipeline for high-throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI-driv...
https://febs.onlinelibrary.wiley.com/doi/full/10.1002/2211-5463.70161
23 days ago
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We're having a great Functional Imaging and Super-Resolution Microscopy course this year again. With engaged students learning about FRAP, FRET, STED, FLIM and many more (not necessarily 4 letter) fluorescent microscopy techniques. But also a really nice talk by Ben Giepmans about ColorEM.
about 2 months ago
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Fun to see a lightsheet image, made at the Erasmus OIC by Lieke Stockman be awarded with the second prize, in the bruker lightsheet image competition.
www.bruker.com/en/products-...
5 months ago
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It's the time of the year (just before holiday) where I can do some random stuff to keep the python muscles warm. Here is a simulation of particles with different movement types in different containers. The bubble like structure was hard to code, but it mostly works.
youtu.be/2YuseN3GGKc
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Particle movement types in different containers
YouTube video by Johan Slotman
https://youtu.be/2YuseN3GGKc
5 months ago
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Sometimes you have to deal with some inevitable truths, like proton beam angle that is fixed. So we flipped a microscope in order to see the effect of proton irradiation on live cells to carefully quantify DNA damage and determine dosage. Read more here:
www.sciencedirect.com/science/arti...
7 months ago
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I think it would be pretty interesting to see something in the color olo.
www.nature.com/articles/d41...
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Brand-new colour created by tricking human eyes with laser
The ‘off-the-charts saturated’ greenish hue — called olo — has been seen by only five study participants.
https://www.nature.com/articles/d41586-025-01252-3
8 months ago
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So lets try bluesky as well for science. I'm an assistant professor focusing on advanced fluorescent imaging and image analysis and part of the Erasmus MC Optical Imaging Centre core facility.
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10 months ago
1
18
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