First manuscript from my lab is now under review! To investigate the effects of modified nucleotide bases commonly used in mRNA therapeutics on RNA 3D structure and function, we synthesized two well characterized RNA with either 5mC or N1m-pseudoU.

First paper of 2024! This was a very fun and highly collaborative project! www.pnas.org/doi/10.1073/...

So I made the bold move to get one of these bad boys so that we can plunge in house ASAP after samples get off the FPLC. I've only ever used vitrobots and Leica GP2 before. Does anyone have any tips for manual plunging or is it just a matter of practice and trial and error? #CryoEM #Vitrification